This research aimed to know about the growth of oil palm seedlings (Elaeis guineensis Jacq.) in pre-nursery stage. This study is conducted on April 9, 2019 to July 17, 2019 in Mr. Widodo’s farming land which is located in Jurug village, Argosari, Sedayu, Bantul. This research used 1 factor, 5 treatments, and 3 repetitions Completely Randomized Design (CRD). The treatment tested is A: (0% OPEFB : 100% sandy soil), B: (25% OPEFB : 75% sandy soil), C: (50% OPEFB : 50% sandy soil), D: (75% OPEFB : 25% sandy soil) dan E: (100% OPEFB : 0% sandy soil). The parameter observed were plant height, number of leaves, crown fresh weight, crown dry weight, root fresh weight, root dry weight, root volume, crown/root ratio, initial media analysis and data analysis. The result from this study showed that sandy soil media and OPEFB compost could not have a good effect on the growth of oil palm seedlings in pre-nursery.